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Residual cells, debris were discarded by centrifugation and CM concentrated with concentricon devices (Millipore, Temecula, CA) with a 3-kDa membrane pore cut-off, which eliminates the residual ALCAR

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Cell viability Resazurin reduction assay Resazurin (Sigma) was added to the cell culture medium (5 g/ml) for a period of at least 30 min

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